Video about Karyotyping

 the following video will show the technique of karyotyping

Excel sheet/ Trainees Baakline- 2016

the following link will represent the G-mails of the trainees

 :https://drive.google.com/file/d/0B7sBq-lVtmIyakZHdzRlb29wS0k/view?usp=sharing

Activity 1: Karyotyping Technique

Karyotyping Technique

1. Click on the following link.

https://www.youtube.com/watch?v=7ShPzzrCetE

2. Then, open your book page 34 and write on the provided drawings supplementary information that you have learned from the YouTube video which might deepen your understanding of this process.

3. In pairs, answer the following questions:

Work alone to answer the following questions:

  1- Which cells are used to perform karyotyping? Why?

 2- State the importance of the following steps:

o Culturing of obtained cells

o Adding Colchicine

o Adding salt

o Spreading cells 

Introduction

                                                                   Karyotype                                                                

A karyotype is an organized profile of a person's chromosomes. To make a karyotype, scientists take a picture of the chromosomes from one nucleated cell; usually a white blood cell, cut the chromosomes out, and arranges them in pairs by size from largest to smallest(with the exception of sex chromosomes), banding pattern, and centromere position. This arrangement helps scientists quickly identify chromosomal alterations that may result in a genetic disorder. Two chromosomes specify gender — XX for female and XY for male. Thus, a karyotype can also reveal the gender of a fetus and test for certain defects through examination of cells from uterine fluid – a procedure called amniocentesis – or through sampling of placental membranes. Moreover, a karyotype can show prospective parents whether they have certain abnormalities that could be passed on to their offspring, or it may be used to learn the cause of a child’s disability.

In this session, participants will investigate how karyotyping is performed in the lab. They will be engaged in several interactive activities that would enhance their understanding of this technique. Participants will infer that chromosomes are the carriers of genetic information by evaluating case histories of patients with different chromosomal set. Additionally, participants will use several technological tools (image editor; desktop publishing software; Google docs; and web sites) to identify common diseases associated with chromosomal abnormalities and share findings with other group members.